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mx3000 multiplex quantitative pcr system  (Agilent technologies)


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    Structured Review

    Agilent technologies mx3000 multiplex quantitative pcr system
    Mx3000 Multiplex Quantitative Pcr System, supplied by Agilent technologies, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mx3000+multiplex+quantitative+pcr+system/pm34864211-49-6-11
    Average 90 stars, based on 1 article reviews
    mx3000 multiplex quantitative pcr system - by Bioz Stars, 2026-09
    90/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Integrative genomic analysis of CREB defines a critical role for transcription factor networks in mediating the fed/fasted switch in liver
    Article Snippet: RNA was reverse transcribed using oligo (dT) and Superscript II reverse transcriptase (Invitrogen). .. The resulting cDNA samples were used as template for qPCR experiments performed with SYBR GreenER qPCR Supermix (Invitrogen) and the SYBR Green program on the Mx3000 Multiplex Quantitative PCR System (Stratagene). ..

    Article Title: Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.
    Article Snippet: .. Quantitative RT-PCR and western blot analysis RT-PCR amplification was performed with the Mx3000 Multiplex Quantitative PCR System (Stratagene Corp, La Jolla, California), SYBR Green I reagent, and the 18S internal control for normalization. ..

    Article Title: Chemokine-Like Factor 1 (CLFK1) Is Over-Expressed in Patients with Atopic Dermatitis
    Article Snippet: The total RNA was extracted from human skin specimens and was reverse-transcribed by a Reverse Transcription Kit (Invitrogen). .. The real-time PCR amplification was performed using an Mx3000 Multiplex Quantitative PCR System (Stratagene Corp, La Jolla, CA), the primers for human CKLF1 were as follows: forward, 5'-TCGCTTCGCAGAACCTACTCA-3', reverse, 5'-TATTTTCGGCTGCACGTTATCC-3'. ..

    Article Title: The Matricellular Protein SPARC Decreases in the Lacrimal Gland At Adulthood and During Inflammation
    Article Snippet: Primer sequences for Sparc (Unique Assay ID qMmuCID0023536) and Gapdh (Unique Assay ID qMmuCED0027497) mRNA were obtained from Bio-Rad. .. Gene expression was measured in a Stratagene Mx3000 Multiplex Quantitative PCR system (Agilent, Santa Clara, CA, USA) with the following parameters: 2 minutes at 95°C, followed by 40 cycles of 5 seconds at 95°C and 30 seconds at 60°C. ..

    Article Title: A novel type lavandulyl flavonoid from Sophora flavescens as potential anti-hepatic injury agent that inhibit TLR2/NF-κB signaling pathway.
    Article Snippet: Ethnopharmacology relevance: Sophora flavescens Aiton, was a crucial source of Traditional Chinese Medicine (TCM) that has benefited human health for hundreds of years.. Alkaloids and flavonoids were the major bioactive constituents from S. flavescens, which had been widely used for liver disease treatment in China.. However, the liver-protective components of flavonoids from S. flavescens and their mechanism of action were not clear.

    Article Title: Improvement of Adipose Macrophage Polarization in High Fat Diet-Induced Obese GHSR Knockout Mice
    Article Snippet: .. PCR was conducted in a 25 μ l volume containing 2.5 ng cDNA, 5mM MgCl 2 , 0.2 mM dNTPs, 0.2 μ M each primer, 1.25 U AmpliTaq Polymerase, and 1 μ l 800x diluted SYBRGreen I stock using the Mx3000 multiplex quantitative PCR system (Stratagene, La Jolla, CA). ..

    Article Title: Evaluation of the relative expression of genes associated with adherence after different hours of co-culture between Streptococcus uberis and MAC-T cells.
    Article Snippet: .. The reactions were performed in a MX3000 Multiplex Quantitative PCR system (Stratagene-Agilent) by using iTaq Universal SRYB Green 2X SuperMix kit (Bio-Rad) in duplicate in two independent experiments. ..

    Article Title: 8,9-Epoxyeicosatrienoic Acid Inhibits Antibody Production of B Lymphocytes in Mice
    Article Snippet: .. Amplification reactions involved the Mx3000 Multiplex Quantitative PCR System (Stratagene, La Jolla, CA). .. Data were analyzed with use of Stratagene Mx3000 software.

    SYBR Green Assay:

    Article Title: Integrative genomic analysis of CREB defines a critical role for transcription factor networks in mediating the fed/fasted switch in liver
    Article Snippet: RNA was reverse transcribed using oligo (dT) and Superscript II reverse transcriptase (Invitrogen). .. The resulting cDNA samples were used as template for qPCR experiments performed with SYBR GreenER qPCR Supermix (Invitrogen) and the SYBR Green program on the Mx3000 Multiplex Quantitative PCR System (Stratagene). ..

    Article Title: Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.
    Article Snippet: .. Quantitative RT-PCR and western blot analysis RT-PCR amplification was performed with the Mx3000 Multiplex Quantitative PCR System (Stratagene Corp, La Jolla, California), SYBR Green I reagent, and the 18S internal control for normalization. ..

    Multiplex Assay:

    Article Title: Integrative genomic analysis of CREB defines a critical role for transcription factor networks in mediating the fed/fasted switch in liver
    Article Snippet: RNA was reverse transcribed using oligo (dT) and Superscript II reverse transcriptase (Invitrogen). .. The resulting cDNA samples were used as template for qPCR experiments performed with SYBR GreenER qPCR Supermix (Invitrogen) and the SYBR Green program on the Mx3000 Multiplex Quantitative PCR System (Stratagene). ..

    Article Title: Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.
    Article Snippet: .. Quantitative RT-PCR and western blot analysis RT-PCR amplification was performed with the Mx3000 Multiplex Quantitative PCR System (Stratagene Corp, La Jolla, California), SYBR Green I reagent, and the 18S internal control for normalization. ..

    Article Title: Chemokine-Like Factor 1 (CLFK1) Is Over-Expressed in Patients with Atopic Dermatitis
    Article Snippet: The total RNA was extracted from human skin specimens and was reverse-transcribed by a Reverse Transcription Kit (Invitrogen). .. The real-time PCR amplification was performed using an Mx3000 Multiplex Quantitative PCR System (Stratagene Corp, La Jolla, CA), the primers for human CKLF1 were as follows: forward, 5'-TCGCTTCGCAGAACCTACTCA-3', reverse, 5'-TATTTTCGGCTGCACGTTATCC-3'. ..

    Article Title: The Matricellular Protein SPARC Decreases in the Lacrimal Gland At Adulthood and During Inflammation
    Article Snippet: Primer sequences for Sparc (Unique Assay ID qMmuCID0023536) and Gapdh (Unique Assay ID qMmuCED0027497) mRNA were obtained from Bio-Rad. .. Gene expression was measured in a Stratagene Mx3000 Multiplex Quantitative PCR system (Agilent, Santa Clara, CA, USA) with the following parameters: 2 minutes at 95°C, followed by 40 cycles of 5 seconds at 95°C and 30 seconds at 60°C. ..

    Article Title: A novel type lavandulyl flavonoid from Sophora flavescens as potential anti-hepatic injury agent that inhibit TLR2/NF-κB signaling pathway.
    Article Snippet: Ethnopharmacology relevance: Sophora flavescens Aiton, was a crucial source of Traditional Chinese Medicine (TCM) that has benefited human health for hundreds of years.. Alkaloids and flavonoids were the major bioactive constituents from S. flavescens, which had been widely used for liver disease treatment in China.. However, the liver-protective components of flavonoids from S. flavescens and their mechanism of action were not clear.

    Article Title: Improvement of Adipose Macrophage Polarization in High Fat Diet-Induced Obese GHSR Knockout Mice
    Article Snippet: .. PCR was conducted in a 25 μ l volume containing 2.5 ng cDNA, 5mM MgCl 2 , 0.2 mM dNTPs, 0.2 μ M each primer, 1.25 U AmpliTaq Polymerase, and 1 μ l 800x diluted SYBRGreen I stock using the Mx3000 multiplex quantitative PCR system (Stratagene, La Jolla, CA). ..

    Article Title: Evaluation of the relative expression of genes associated with adherence after different hours of co-culture between Streptococcus uberis and MAC-T cells.
    Article Snippet: .. The reactions were performed in a MX3000 Multiplex Quantitative PCR system (Stratagene-Agilent) by using iTaq Universal SRYB Green 2X SuperMix kit (Bio-Rad) in duplicate in two independent experiments. ..

    Article Title: 8,9-Epoxyeicosatrienoic Acid Inhibits Antibody Production of B Lymphocytes in Mice
    Article Snippet: .. Amplification reactions involved the Mx3000 Multiplex Quantitative PCR System (Stratagene, La Jolla, CA). .. Data were analyzed with use of Stratagene Mx3000 software.

    Quantitative RT-PCR:

    Article Title: Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.
    Article Snippet: .. Quantitative RT-PCR and western blot analysis RT-PCR amplification was performed with the Mx3000 Multiplex Quantitative PCR System (Stratagene Corp, La Jolla, California), SYBR Green I reagent, and the 18S internal control for normalization. ..

    Western Blot:

    Article Title: Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.
    Article Snippet: .. Quantitative RT-PCR and western blot analysis RT-PCR amplification was performed with the Mx3000 Multiplex Quantitative PCR System (Stratagene Corp, La Jolla, California), SYBR Green I reagent, and the 18S internal control for normalization. ..

    Reverse Transcription Polymerase Chain Reaction:

    Article Title: Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.
    Article Snippet: .. Quantitative RT-PCR and western blot analysis RT-PCR amplification was performed with the Mx3000 Multiplex Quantitative PCR System (Stratagene Corp, La Jolla, California), SYBR Green I reagent, and the 18S internal control for normalization. ..

    Amplification:

    Article Title: Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.
    Article Snippet: .. Quantitative RT-PCR and western blot analysis RT-PCR amplification was performed with the Mx3000 Multiplex Quantitative PCR System (Stratagene Corp, La Jolla, California), SYBR Green I reagent, and the 18S internal control for normalization. ..

    Article Title: Chemokine-Like Factor 1 (CLFK1) Is Over-Expressed in Patients with Atopic Dermatitis
    Article Snippet: The total RNA was extracted from human skin specimens and was reverse-transcribed by a Reverse Transcription Kit (Invitrogen). .. The real-time PCR amplification was performed using an Mx3000 Multiplex Quantitative PCR System (Stratagene Corp, La Jolla, CA), the primers for human CKLF1 were as follows: forward, 5'-TCGCTTCGCAGAACCTACTCA-3', reverse, 5'-TATTTTCGGCTGCACGTTATCC-3'. ..

    Article Title: A novel type lavandulyl flavonoid from Sophora flavescens as potential anti-hepatic injury agent that inhibit TLR2/NF-κB signaling pathway.
    Article Snippet: Ethnopharmacology relevance: Sophora flavescens Aiton, was a crucial source of Traditional Chinese Medicine (TCM) that has benefited human health for hundreds of years.. Alkaloids and flavonoids were the major bioactive constituents from S. flavescens, which had been widely used for liver disease treatment in China.. However, the liver-protective components of flavonoids from S. flavescens and their mechanism of action were not clear.

    Article Title: 8,9-Epoxyeicosatrienoic Acid Inhibits Antibody Production of B Lymphocytes in Mice
    Article Snippet: .. Amplification reactions involved the Mx3000 Multiplex Quantitative PCR System (Stratagene, La Jolla, CA). .. Data were analyzed with use of Stratagene Mx3000 software.

    Control:

    Article Title: Targeting macrophage TFEB-14-3-3 epsilon Interface by naringenin inhibits abdominal aortic aneurysm.
    Article Snippet: .. Quantitative RT-PCR and western blot analysis RT-PCR amplification was performed with the Mx3000 Multiplex Quantitative PCR System (Stratagene Corp, La Jolla, California), SYBR Green I reagent, and the 18S internal control for normalization. ..

    Gene Expression:

    Article Title: The Matricellular Protein SPARC Decreases in the Lacrimal Gland At Adulthood and During Inflammation
    Article Snippet: Primer sequences for Sparc (Unique Assay ID qMmuCID0023536) and Gapdh (Unique Assay ID qMmuCED0027497) mRNA were obtained from Bio-Rad. .. Gene expression was measured in a Stratagene Mx3000 Multiplex Quantitative PCR system (Agilent, Santa Clara, CA, USA) with the following parameters: 2 minutes at 95°C, followed by 40 cycles of 5 seconds at 95°C and 30 seconds at 60°C. ..

    Polymerase Chain Reaction:

    Article Title: Improvement of Adipose Macrophage Polarization in High Fat Diet-Induced Obese GHSR Knockout Mice
    Article Snippet: .. PCR was conducted in a 25 μ l volume containing 2.5 ng cDNA, 5mM MgCl 2 , 0.2 mM dNTPs, 0.2 μ M each primer, 1.25 U AmpliTaq Polymerase, and 1 μ l 800x diluted SYBRGreen I stock using the Mx3000 multiplex quantitative PCR system (Stratagene, La Jolla, CA). ..



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